4 Mai 2017: Séminaire du Dr.T. Kobayashi -Strasbourg     


Invité par le Dr. Hullin-Matsuda, (Equipe 4  CarMeN), le Dr.Toshihide Kobayashi (CNRS UMR 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie – Université de Strasbourg, ILLKIRCH) a présenté un séminaire CarMeN sur le site de l’INSA le 4 mai en salle de réunion  de l’IMBL (15 avenue Jean Capelle, Villeurbanne)

Titre:  « Acute accumulation of free cholesterol induces fusion of ER and lipid droplets« 

Résumé:Dysregulated hepatic cholesterol homeostasis with free cholesterol accumulation in the liver is relevant to the pathogenesis of nonalcoholic steatohepatitis, contributing to the chronicity of liver toxicity. Here we examined the effect of free cholesterol accumulation on the morphology and biochemical properties of lipid droplets (LDs) in cultured hepatocytes. Acute free cholesterol accumulation induced the fusion of LDs, followed by degradation of the coat protein of LDs, perilipin 2 (PLIN2; also called adipophilin or adipose differentiation–related protein), and association of apolipoprotein B 100 (ApoB 100) to LDs. The degradation of PLIN2 was inhibited by inhibitors of ubiquitination, autophagy, and protein synthesis. The results indicate that association of ApoB 100 with LDs is dependent on the activity of low–molecular weight GTP-binding protein Rab18 and highlight the role of LDs as targets of free cholesterol toxicity in hepatocytes.Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton I, Kobayashi T. (2016) Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol Biol Cell 27, 3293-3304