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Image Microscopy

Microscopy

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The technical platform is equipped with 4 microscopes and 1 workstation dedicated to image analysis.

  • The A1R + is a powerful, fully automated Nikon confocal imaging system capable of capturing high quality confocal images of cells and molecular events at high speed and with increased sensitivity.
  • The Leica system incorporates a light source for millisecond excitation wavelength switching, a high-sensitivity camera, tools perfect for FRET sensor or ratiometric probe analysis, and software designed for automated acquisition and dual-wavelength measurements of intracellular ions.
  • The MetaXpress Pico is an automated cell imaging system. It combines high-resolution imaging with powerful analysis. Whether you use fluorescence imaging or brightfield analysis, the automated imager offers a wide range of preconfigured protocols for cell-based assays. With features such as 2D deconvolution, Live Preview and 3-color quantification at multiple wavelengths. MetaXpress image acquisition and analysis software with high information content is included.
  • The IonOptix is a system that allows the simultaneous acquisition of contraction and calcium variations of cardiomyocytes using chemical probes (Fura2) or FRET sensors.

The platform is located at the Groupement Hospitalier Est, 59 Boulevard Pinel, 69500 BRON, Bâtiment B13.

Our publications

2024

  • Badawi S, Leboullenger C, Chourrout M, Gouriou Y, Paccalet A, Pillot B, Augeul L, Bolbos R, Bongiovani A, Mewton N, Bochaton T, Ovize M, Tardivel M, Kurdi M, Canet-Soulas E, Da Silva CC, Bidaux G. Oxidation-reduction imaging of myoglobin reveals two-phase oxidation in the reperfused myocardium. Basic Res Cardiol. 2024 Mar 18. doi: 10.1007/s00395-024-01040-6.

2023

  • Gonnot F, Boulogne L, Brun C, Dia M, Gouriou Y, Bidaux G, Chouabe C, Crola Da Silva C, Ducreux S, Pillot B, Kaczmarczyk A, Leon C, Chanon S, Perret C, Sciandra F, Dargar T, Gache V, Farhat F, Sebbag L, Bochaton T, Thibault H, Ovize M, Paillard M, Gomez L. SERCA2 phosphorylation at serine 663 is a key regulator of Ca2+ homeostasis in heart diseases. Nat Commun. 2023 Jun 8;14(1):3346. doi: 10.1038/s41467-023-39027-x.
  • Gouriou Y, Gonnot F, Wehbi M, Brun C, Gomez L, Bidaux G. High-sensitivity calcium biosensor on the mitochondrial surface reveals that IP3R channels participate in the reticular Ca2+ leak towards mitochondria. PLoS One. 2023 Jun 9;18(6):e0285670. doi: 10.1371/journal.pone.0285670. eCollection 2023.
  • Tessier N, Ducrozet M, Dia M, Badawi S, Chouabe C, Crola Da Silva C, Ovize M, Bidaux G, Van Coppenolle F, Ducreux S. TRPV1 Channels Are New Players in the Reticulum-Mitochondria Ca2+ Coupling in a Rat Cardiomyoblast Cell Line. Cells. 2023 Sep 20;12(18):2322. doi: 10.3390/cells12182322.
  • Martin LS, Josset-Lamaugarny A, El Jammal T, Ducreux S, Chevalier FP, Fromy B. Aging is associated with impaired triggering of TRPV3-mediated cutaneous vasodilation: a crucial process for local heat exposure. Geroscience. 2023 Oct 19. doi: 10.1007/s11357-023-00981-5.

2022

  • Beaulant, A., Dia, M., Pillot, B., Chauvin, M. A., Ji-Cao, J., Durand, C., Bendridi, N., Chanon, S., Vieille-Marchiset, A., Da Silva, C. C., Patouraux, S., Anty, R., Iannelli, A., Tran, A., Gual, P., Vidal, H., Gomez, L., Paillard, M. and Rieusset, J. Endoplasmic reticulum-mitochondria miscommunication is an early and causal trigger of hepatic insulin resistance and steatosis J Hepatol. 2022 Sep;77(3). doi: 10.1016/j.jhep.2022.03.017.
  • Raynard, C., Tessier, N., Huna, A., Warnier, M., Flaman, J. M., Van Coppenolle, F., Ducreux, S., Martin, N. and Bernard, D. Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca(2+) Levels in Senescent Cells Int J Mol Sci. 2022 Aug 19;23(16). doi: 10.3390/ijms23169376.
  • Raynard, C., Ma, X., Huna, A., Tessier, N., Massemin, A., Zhu, K., Flaman, J. M., Moulin, F., Goehrig, D., Medard, J. J., Vindrieux, D., Treilleux, I., Hernandez-Vargas, H., Ducreux, S., Martin, N. and Bernard, D. NF-κB-dependent secretome of senescent cells can trigger neuroendocrine transdifferentiation of breast cancer cells Aging Cell. 2022 Jul;21(7). doi: 10.1111/acel.13632.

2020

  • Al-Mawla R, Ducrozet M, Tessier N, Païta L, Pillot B, Gouriou Y, Villedieu C, Harhous Z, Paccalet A, Crola Da Silva C, Ovize M, Bidaux G, Ducreux S, Van Coppenolle F. Acute Induction of Translocon-Mediated Ca2+ Leak Protects Cardiomyocytes Against Ischemia/Reperfusion Injury. Cells. 2020 May 25;9(5):1319. doi: 10.3390/cells9051319.
  • Bosson C, Rendu J, Pelletier L, Abriat A, Chatagnon A, Brocard J, Brocard J, Figarella-Branger D, Ducreux S, van Coppenolle F, Sagui E, Marty I, Roux-Buisson N, Faure J. Variations in the TRPV1 gene are associated to exertional heat stroke. J Sci Med Sport. 2020 Nov;23(11):1021-1027. doi: 10.1016/j.jsams.2020.04.018.
  • Dia M, Gomez L, Thibault H, Tessier N, Leon C, Chouabe C, Ducreux S, Gallo-Bona N, Tubbs E, Bendridi N, Chanon S, Leray A, Belmudes L, Couté Y, Kurdi M, Ovize M, Rieusset J, Paillard M. Reduced reticulum-mitochondria Ca2+ transfer is an early and reversible trigger of mitochondrial dysfunctions in diabetic cardiomyopathy. Basic Res Cardiol. 2020 Nov 30;115(6):74. doi: 10.1007/s00395-020-00835-7.
  • Gouriou Y, Alam MR, Harhous Z, Crola Da Silva C, Baetz DB, Badawi S, Lefai E, Rieusset J, Durand A, Harisseh R, Gharib A, Ovize M, Bidaux G. ANT2-Mediated ATP Import into Mitochondria Protects against Hypoxia Lethal Injury. Cells. 2020 Nov 25;9(12):2542. doi: 10.3390/cells9122542.

2019

  • Dingreville F, Panthu B, Thivolet C, Ducreux S, Gouriou Y, Pesenti S, Chauvin MA, Chikh K, Errazuriz-Cerda E, Van Coppenolle F, Rieusset J, Madec AM. Differential Effect of Glucose on ER-Mitochondria Ca2+ Exchange Participates in Insulin Secretion and Glucotoxicity-Mediated Dysfunction of β-Cells. Diabetes. 2019 Sep;68(9):1778-1794. doi: 10.2337/db18-1112.
  • Beringer A, Gouriou Y, Lavocat F, Ovize M, Miossec P. Blockade of Store-Operated Calcium Entry Reduces IL-17/TNF Cytokine-Induced Inflammatory Response in Human Myoblasts. Front Immunol. 2019 Jan 14;9:3170. doi: 10.3389/fimmu.2018.03170.
  • Harhous Z, Badawi S, Bona NG, Pillot B, Augeul L, Paillard M, Booz GW, Canet-Soulas E, Ovize M, Kurdi M, Bidaux G. Critical appraisal of STAT3 pattern in adult cardiomyocytes. J Mol Cell Cardiol. 2019 Jun;131:91-100. doi: 10.1016/j.yjmcc.2019.04.021.

2018

  • Delcroix V, Mauduit O, Tessier N, Montillaud A, Lesluyes T, Ducret T, Chibon F, Van Coppenolle F, Ducreux S, Vacher P. The Role of the Anti-Aging Protein Klotho in IGF-1 Signaling and Reticular Calcium Leak: Impact on the Chemosensitivity of Dedifferentiated Liposarcomas. Cancers (Basel). 2018 Nov 14;10(11):439. doi: 10.3390/cancers10110439.
  • Bidaux G, Gordienko D, Shapovalov G, Farfariello V, Borowiec AS, Iamshanova O, Lemonnier L, Gueguinou M, Guibon R, Fromont G, Paillard M, Gouriou Y, Chouabe C, Dewailly E, Gkika D, López-Alvarado P, Carlos Menéndez J, Héliot L, Slomianny C, Prevarskaya N. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+2+ transfer. Biochim Biophys Acta Mol Cell Res. 2018 Jul;1865(7):981-994. doi: 10.1016/j.bbamcr.2018.04.007.
  • Tubbs E, Chanon S, Robert M, Bendridi N, Bidaux G, Chauvin MA, Ji-Cao J, Durand C, Gauvrit-Ramette D, Vidal H, Lefai E, Rieusset J. Disruption of Mitochondria-Associated Endoplasmic Reticulum Membrane (MAM) Integrity Contributes to Muscle Insulin Resistance in Mice and Humans. Diabetes. 2018 Apr;67(4):636-650. doi: 10.2337/db17-0316.

2016

  • Jalabert A, Vial G, Guay C, Wiklander OP, Nordin JZ, Aswad H, Forterre A, Meugnier E, Pesenti S, Regazzi R, Danty-Berger E, Ducreux S, Vidal H, El-Andaloussi S, Rieusset J, Rome S. Exosome-like vesicles released from lipid-induced insulin-resistant muscles modulate gene expression and proliferation of beta recipient cells in mice. Diabetologia. 2016 May;59(5):1049-58. doi: 10.1007/s00125-016-3882-y.
  • Cassel R, Ducreux S, Alam MR, Dingreville F, Berlé C, Burda-Jacob K, Chauvin MA, Chikh K, Païta L, Al-Mawla R, Crola Da Silva C, Rieusset J, Thivolet C, Van Coppenolle F, Madec AM. Protection of Human Pancreatic Islets from Lipotoxicity by Modulation of the Translocon. PLoS One. 2016 Feb 10;11(2):e0148686. doi: 10.1371/journal.pone.0148686.
  • Fouqué A, Lepvrier E, Debure L, Gouriou Y, Malleter M, Delcroix V, Ovize M, Ducret T, Li C, Hammadi M, Vacher P, Legembre P. The apoptotic members CD95, BclxL, and Bcl-2 cooperate to promote cell migration by inducing Ca(2+) flux from the endoplasmic reticulum to mitochondria. Cell Death Differ. 2016 Oct;23(10):1702-16. doi: 10.1038/cdd.2016.61.

 

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